Figure 7.

mRNA of HTRA2 in cultured cells increases upon etoposide treatment or p53 expression. (A) Northern blot analysis of HTRA2 mRNA induction upon etoposide treatment. Total RNA was harvested from HeLa cells without or with etoposide treatment for the indicated amount of time. mRNA of HTRA2 or GAPDH genes was detected using ³²P-labeled cDNA probes, quantified by phosphorimager. The fold of induction was normalized against the GAPDH level. (B) RT–PCR analysis of HTRA2 and β-actin mRNA from HeLa cells or H1299 cells transfected with p53 expression vector or their respective control (mock-transfected) cells. Intensity of the radioactive species was quantified by PhosphorImager. The fold of induction was normalized against β-actin. These data are representative results from two independent experiments.