Figure 5.

TCR- or second messenger-mediated changes in P2Y₂ or P2X₁ receptor mRNA expression. (A) TCR-mediated changes in P2Y₂ or P2X₁ receptor mRNA expression. Thymocytes from 6-week-old male or female DBA/2 mice were incubated with immobilized anti-CD3 (2C11) mAb (10 μg/ml) for indicated lengths of time. P2Y₂ or P2X₁ receptor mRNA levels were determined by quantitative or semiquantitative RT-PCR. The RT-PCR results were corrected for heteroduplex formation and normalized to β-actin mRNA expression determined by semiquantitative RT-PCR, as described. Data shown here is a representative result from male mice. (B) Second messenger-mediated changes in P2Y₂ receptor mRNA expression. Thymocytes from 6-week-old male DBA/2 mice were incubated for indicated lengths of time with 15 nM PMA and/or 0.3 μM ionomycin. P2Y₂ receptor and GAPDH mRNA levels were determined by semiquantitative RT-PCR. The RT-PCR results were corrected for heteroduplex formation and normalized to GAPDH mRNA expression, as described.