Figure 7.

HSF1 redox activation is required for Hsp induction in response to heat shock and H₂O₂ stress. hsf^−/− MEF stable cells lines with either vector or expressing the wild-type and indicated HSF1 alleles were treated under control (37°C), heat shock (42°C for 1 h; left panel), or H₂O₂ (500 μM for 24 h; right panel) conditions. After a 24-h recovery, total cell extracts were prepared, then fractionated by SDS-PAGE, and Hsp70, Hsp27, and actin were detected by immunoblotting with the respective protein-specific antibody.