Figure 4.

CpG methylation analyses. (A) Southern blot analysis of genomic DNA extracted from various organs dissected from neonatal offspring of reciprocal crosses between 142* and SD7 mice. The parental alleles were discriminated by exploiting a polymorphic BstXI site, as indicated in A. (B) Bisulphite sequencing analyses of the mutated H19 ICR allele between −2836 to −2479 bp from the H19 transcriptional start site, covering the mutated CTCF target site #3. The wild-type and mutant H19 ICR alleles were discriminated on the basis of the introduced point mutations within CTCF target site #3. The tissue type and the sex of the parent transmitting the mutant allele is indicated above each scheme. Filled boxes indicate methylated CpGs and no box indicates no methylation at the positions indicated above each row.