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. 2003 Mar 15;17(6):738–747. doi: 10.1101/gad.1065703

Figure 1.

Figure 1

Targeted disruption of the mouse Tpit gene. (A) Homologous recombination between the mouse Tpit gene (top) and the targeting vector (bottom) will result in replacement of almost all of the coding region by a lacZ coding gene. K, KpnI; B, BamHI. (B) PCR assay for genotyping using oligonucleotides identified by arrows in A. Wild-type and Tpit−/− PCR products are 350 and 750 bp, respectively. (C) Tpit immunohistochemical analysis of pituitaries from wild-type and Tpit−/− mice showing absence of Tpit protein in E14.5 mutant embryo and adult. (D) LacZ staining of pituitary from Tpit+/− mouse showing expression of β-galactosidase throughout intermediate lobe (IL) melanotroph cells and in a subset of anterior lobe (AL) cells (corticotrophs), whereas no expression is detected in posterior lobe (PL).