Figure 4.

Repression of gonadotroph differentiation in transgenic mice expressing Tpit under control of αGSU promoter. Expression of marker genes was assessed by immunohistochemistry on sections of pituitaries from wild-type or transgenic mice (four transgenics showed a similar phenotype). A slight increase in the number of anterior lobe (AL) Tpit-positive (A) and POMC-positive (B) cells is observed in transgenic pituitary, whereas a decrease of αGSU (C) and βFSH (F) expression is observed. LHβ is no longer detectable (E), whereas βTSH (D) expression appears to be relatively normal. The number of SF1-positive cells (G) is decreased in the transgenic pituitaries.