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. 2003 Mar 15;17(6):748–758. doi: 10.1101/gad.1068203

Figure 2.

Figure 2

Wild-type and alanine-substituted CTD heptapeptide repeats bind CycT1. (A) GST pull-down. Binding reactions (lanes 1–8) were performed as indicated by plus signs above the autoradiograph. Arrows to the right point to the wild-type Lex.CTD(SS)16 and the mutant Lex.CTD(AA)17 chimeras. (B, Left) The input of Lex.CTD fusion proteins from IVT. Plus signs above the autoradiograph indicate the Lex.CTD fusion proteins. Inputs represent 10% of the amounts that were used for GST pull-downs. Curved lines to the right point to the Lex.CTD fusion proteins. (Right) The input of GST and GST.CycT1 fusion protein.