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. 2003 Mar 15;17(6):748–758. doi: 10.1101/gad.1068203

Figure 4.

Figure 4

PIE-1 requires the CTD-like repeat to bind CycT1. (A) PIE-1 binds CycT1. COS cell lysates expressing the Flag epitope-tagged wild-type PIE-1 fusion protein (Flag.PIE-1, lanes 1,2) or the Flag epitope tag alone (Flag, lane 3) were incubated with the GST.CycT1 fusion protein (lanes 1,3) or GST alone (lane 2) as indicated by plus signs above the Western blot. The arrow to the right indicates the presence of Flag.PIE1. (B) PIE-1 binds CycT1 through its CTD-like repeat. (Left) Binding reactions (lanes 1–8) that were performed as indicated by plus signs above the autoradiograph. Arrows to the right point to the bound Flag epitope-tagged PIE-1 proteins. (Right) Inputs of Flag epitope-tagged PIE-1 fusion proteins from IVT as indicated by plus signs above the autoradiograph. Inputs represent 10% of the amounts that were used for GST pull-downs. The curved lines to the right point to the Flag epitope-tagged PIE-1 fusion proteins.