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Analysis of replication timing by DNA FISH. The top three panels show representative ES cell nuclei, which were hybridized with a probe specific for the Igf2R locus. The nucleus in the left panel shows two unreplicated alleles (SS), the middle panel shows a nucleus with one replicated and one unreplicated allele (SD), and the right panel shows a nucleus in which both alleles are replicated (DD). The bottom three panels show the same nuclei stained with anti-BrdU antibody.
