Table 1.
Replication timing analysis in wild-type and mutant ES cell lines
| Imprinted loci
|
Wildtype
|
Dnmt1−/−
|
3a−/−, 3b−/−
|
3Lmat−/−/+
|
Parth. ♀♀
|
|---|---|---|---|---|---|
| H19pat | 37% | 39% | 38% | 36% | 20% |
| Igf2pat | 34% | 28% | 40% | 39% | 18% |
| Snrpnmat | 40% | 38% | 39% | 40% | 17% |
| Igf2rmat | 29% | 34% | 41% | 39% | 13% |
| Kvlqt1mat | 32% | 41% | 36% | 33% | 12% |
| P57kip2mat | 34% | 31% | N.D. | 37% | 15% |
| Not imprinted | |||||
| L23mrp | 12% | 13% | 13% | 16% | 18% |
| a-globin | 18% | 19% | N.D. | 16% | 15% |
Relative amount of BrdU positive nuclei, with one replicated and one unreplicated allele (SD) in wildtype, Dnmt1−/−, Dnmt3a−/−b−/−, Dnmt3L(mat−/−)/+ and parthenogenetic ES cells, for several imprinted and nonimprinted loci (pat: Paternally imprinted methylation mark, mat: Maternally imprinted methylation mark).