Table 1.

Transgenes containing daf-28 and ins genes

Gene	Size of 5′-flanking region (kb)	Size of 3′-flanking region (kb)	Sequences of the forward primer	Sequences of the reverse primer
daf-28	3.3	0.45	TTCCACCATTGGTAGGATAGTTGGTTGCCG	TAGAGGAAGAGCGGGGGGGGGAGATC
ins-2	4.0	0.16	TTGTAACCTATCGATTAATGTTCTCAATC	CGCATTGATATCATATGATACATTACGTGC
ins-4	5.5	0.11	GCTGATCGGTCGCTTAGAATTG	AGGAATTGGTTGCACATGG
ins-6a	1.7	0.12	CATTTGACTAATTGGATCCCAG	TTCGCATGGAAATAGACTTCCG
ins-7a	0.66	0.18	CTATTCATCCATCCTTGCTTCC	GAAGGTTGAGATTGTTTCAAGC
ins-9a	1.2	0.15	GACGAGCAGGCACAAGAATAAACG	ATATCAGTATAAATTATTTAACCTGAACCG
ins-17	3.9	0.57	GGTCGACTTCCAGAATTATGATAAGTG	CAAGCATGATTGGTGTGGACATAC
ins-21	3.0	2.2	CCGAAACATTCATCATTCCGATAC	TGATCAACAAGGTTTATTGAACGG
ins-22a	2.2	1.6	as above	as above
ins-23a	1.6	0.14	as above	as above

^aMaximum lengths of promoter sequences were included without including the upstream genes.