Figure 5.

Defective cell growth of tcasp8^−/− T-cells. (A) [³H]Thymidine incorporation in tcasp8^−/− and control purified T-cells after 48 or 72 h of stimulation with anti-CD3 (2 or 5 μg/mL) with or without costimulation by anti-CD28 (2 μg/mL) or IL-2 (100 U/mL). Representative experiments are shown. (B) [³H]Thymidine incorporation in tcasp8^−/− and control purified T-cells after stimulation with PMA and ionomycin (concentrations in nanograms per milliliter). Representative experiments are shown. (C) [³H]Thymidine incorporation in B-cells purified from tcasp8^−/− and control mice after 48 or 72 h of activation with various stimuli. (D) The proportion of T-cells in G₀/G₁, S, and G₂/M phases were similar in control and tcasp8^−/− backgrounds after anti-CD3 and anti-CD28 stimulation. The larger G₁ subpopulation in the cycling tcasp8^−/− T-cells at 48 and 72 h after stimulation indicates decreased survival of stimulated tcasp8^−/− T-cells. G₀/G₁, S, and G₂/M are calculated from the total number of cycling T-cells. Sub-G₁ values represent the percentage of the total number of all cells cycling or dead.