Figure 3.

Confirmation of ICE1 gene cloning by expressing the dominant ice1 mutant allele in wild-type plants. (A) Expression in wild type of a genomic fragment containing the ice1 mutation recapitulates the ice1 mutant phenotype. Seven-day-old seedlings of the wild type, ice1, and wild type transformed with the mutant ice1 gene grown on MS agar medium were subjected to luminescence imaging after 12 h of cold (0°C) stress. (B) Quantitation of CBF3–LUC bioluminescence levels in wild type (WT), ice1 and wild type transformed with the mutant ice1 gene after 12 h of cold (0°C) stress.