Figure 5.

Expression of the ICE1 gene and subcellular localization of the ICE1 protein. (A) ICE1 promoter-driven GUS expression pattern in a wild-type seedling. (B) ICE1 promoter-GUS expression in different plant tissues, and the corresponding ICE1 transcript levels as determined by RT–PCR analysis. The tubulin gene was used as an internal control in the RT–PCR. (C) RNA blot analysis of ICE1 expression in wild-type seedlings under various abiotic stresses. Plants with the following treatments are shown: control, MS salt only; NaCl, 300 mM NaCl for 5 h; ABA, 100 μM abscisic acid for 3 h; Cold, 0°C for 2 h; Dehydration, air drying for 30 min. (D) Localization of GFP–ICE1 fusion protein in the nucleus. Panels a–c show confocal images of root cells in GFP–ICE1 transgenic plants, and panel d shows the location of nuclei as indicated by propidium stain.