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EcoRI endonuclease expression experiment. All strains were grown at 20°C on medium containing glucose and then streaked onto medium containing galactose and incubated at 20°C. (A) hdf1Δ null strain (BRY1) transformed with the EcoRI-producing plasmid YCpGalRIb. (B) hdf1Δ null strain (BRY1) transformed with the control vector. (C) Isogenic wild-type cells (BRY4) transformed with the EcoRI plasmid, YCpGalRIb.
