Skip to main content
. 2003 May 15;17(10):1293–1307. doi: 10.1101/gad.1079403

Table 1.

Strains and plasmids

Strain
Genotypea
Source
VH1000 MG1655 lacZ lacI pyrE+ Gaal et al. 1997
NK5031 lacM5265 NalR SupF Gaal et al. 1989
RLG957 NK5031/λ rrnB Pl (−61 to +50)-lacZ Ross et al. 1990
RLG4547 NK5031/λ rrnB Pl (−66 to +50; proximal UP 4547)-lacZ Estrem et al. 1999
RLG4549 NK5031/λ rrnB Pl (−66 to +50; proximal UP 4549)-lacZ Estrem et al. 1999
RLG2258 NK5031/λ system II rrnB Pl (−41 to +50)-lacZ Rao et al. 1994
RLG4755 VH1000/λ system II rrnB Pl (−41 to +50)-lacZ This work
RLG5643 VH1000/λ system II rrnB Pl (−41 to +50)-lacZ ptrp-rpoD This work
RLG5417 VH1000/λ rrnB Pl (−66 to +50; proximal UP 4547)-lacZ This work
RLG5601 VH1000/λ rrnB Pl (−66 to +50; proximal UP 4547)-lacZ ptrp-rpoD This work
RLG5600 VH1000/λ rrnB Pl (−66 to +50; full consensus UP 4192)-lacZ This work
RLG5602 VH1000/λ rrnB Pl (−66 to +50; full consensus UP 4192)-lacZ ptrp-rpoD This work



Plasmid Description Source



pRLG770 Transcription Vector Ross et al. 1990
pRLG4210 rrnB Pl (−66 to +50, with SUB, an upstream sequence lacking UP element function) in pRLG770 Estrem et al. 1999
pRLG4213 rrnB Pl (−66 to +50, with selected proximal subsite UP element 4547) in pRLG770 Estrem et al. 1999
pRLG3289 rrnB Pl (−66 to +50, with selected proximal subsite UP element 4549) in pRLG770 Ross et al. 2001
pRLG3278 rrnB Pl (−66 to +50, with consensus full UP element 4192) in pRLG770 Estrem et al. 1998
pRLG4238 rrnB Pl (−66 to +50, with native UP element) in pRLG770 Estrem et al. 1998
pRLG3266 rrnD Pl (−60 to +1, with native UP element) in pRLG770 Ross et al. 1998
pRLG3267 rrnD Pl (−41 to +1); lacking an UP element) in pRLG770 Ross et al. 1998
pRLG593 placUV5 (−60 to +39) in pRLG770 Ross et al. 1990
pGEX-2T σ70 GST-σ70 (8-613) fusion pasmid, with wild-type or region 4.2 alanine substitutions Lonetto et al. 1998
pRLG4734 pGEX-2Tσ70 (8–613, L598A) This work
pETHis6-σ70 pET28 with wild-type σ70 Marr and Roberts 1997
pRLG3293 pETHis6- σ70 K593A This work
pRLG3295 pETHis6- σ70 K597A This work
pRLG3296 pETHis6- σ70 R603A This work
pHTf1α pHTf1 with wild-type and α273–329 alanine substitutions Gaal et al. 1996; Kainz and Gourse 1998; Tang et al. 1994
pREIIα pREII with wild-type or α255–271 alanine substitutions Tang et al. 1994; Gaal et al. 1996
a

λ Prophage carrying promoter–lacZ fusions are system I (Rao et al. 1994) unless otherwise indicated. 

HHS Vulnerability Disclosure