Table 1.
Strains and plasmids
Strain
|
Genotypea
|
Source
|
---|---|---|
VH1000 | MG1655 lacZ lacI pyrE+ | Gaal et al. 1997 |
NK5031 | lacM5265 NalR SupF | Gaal et al. 1989 |
RLG957 | NK5031/λ rrnB Pl (−61 to +50)-lacZ | Ross et al. 1990 |
RLG4547 | NK5031/λ rrnB Pl (−66 to +50; proximal UP 4547)-lacZ | Estrem et al. 1999 |
RLG4549 | NK5031/λ rrnB Pl (−66 to +50; proximal UP 4549)-lacZ | Estrem et al. 1999 |
RLG2258 | NK5031/λ system II rrnB Pl (−41 to +50)-lacZ | Rao et al. 1994 |
RLG4755 | VH1000/λ system II rrnB Pl (−41 to +50)-lacZ | This work |
RLG5643 | VH1000/λ system II rrnB Pl (−41 to +50)-lacZ ptrp-rpoD | This work |
RLG5417 | VH1000/λ rrnB Pl (−66 to +50; proximal UP 4547)-lacZ | This work |
RLG5601 | VH1000/λ rrnB Pl (−66 to +50; proximal UP 4547)-lacZ ptrp-rpoD | This work |
RLG5600 | VH1000/λ rrnB Pl (−66 to +50; full consensus UP 4192)-lacZ | This work |
RLG5602 | VH1000/λ rrnB Pl (−66 to +50; full consensus UP 4192)-lacZ ptrp-rpoD | This work |
Plasmid | Description | Source |
pRLG770 | Transcription Vector | Ross et al. 1990 |
pRLG4210 | rrnB Pl (−66 to +50, with SUB, an upstream sequence lacking UP element function) in pRLG770 | Estrem et al. 1999 |
pRLG4213 | rrnB Pl (−66 to +50, with selected proximal subsite UP element 4547) in pRLG770 | Estrem et al. 1999 |
pRLG3289 | rrnB Pl (−66 to +50, with selected proximal subsite UP element 4549) in pRLG770 | Ross et al. 2001 |
pRLG3278 | rrnB Pl (−66 to +50, with consensus full UP element 4192) in pRLG770 | Estrem et al. 1998 |
pRLG4238 | rrnB Pl (−66 to +50, with native UP element) in pRLG770 | Estrem et al. 1998 |
pRLG3266 | rrnD Pl (−60 to +1, with native UP element) in pRLG770 | Ross et al. 1998 |
pRLG3267 | rrnD Pl (−41 to +1); lacking an UP element) in pRLG770 | Ross et al. 1998 |
pRLG593 | placUV5 (−60 to +39) in pRLG770 | Ross et al. 1990 |
pGEX-2T σ70 | GST-σ70 (8-613) fusion pasmid, with wild-type or region 4.2 alanine substitutions | Lonetto et al. 1998 |
pRLG4734 | pGEX-2Tσ70 (8–613, L598A) | This work |
pETHis6-σ70 | pET28 with wild-type σ70 | Marr and Roberts 1997 |
pRLG3293 | pETHis6- σ70 K593A | This work |
pRLG3295 | pETHis6- σ70 K597A | This work |
pRLG3296 | pETHis6- σ70 R603A | This work |
pHTf1α | pHTf1 with wild-type and α273–329 alanine substitutions | Gaal et al. 1996; Kainz and Gourse 1998; Tang et al. 1994 |
pREIIα | pREII with wild-type or α255–271 alanine substitutions | Tang et al. 1994; Gaal et al. 1996 |
λ Prophage carrying promoter–lacZ fusions are system I (Rao et al. 1994) unless otherwise indicated.