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. 2003 May 15;17(10):1225–1239. doi: 10.1101/gad.1081203

Figure 5.

Figure 5

LIN-5 and GPR-1/GPR-2 enrichment at the P2/EMS boundary requires mes-1 and src-1. (A–D) Merged images, showing staining of LIN-5 (red), GPR-1/GPR-2 (green), and DNA (DAPI, blue). LIN-5 and GPR-1/GPR-2 are enriched between P2 and EMS (arrow marked 3) in wild-type (A) and mom-5(zu193) (B) embryos, but not in mes-1(bn74) (C) and src-1(RNAi) (D) embryos. (E–H) Measurements of GPR-1/GPR-2 relative fluorescence intensity for the AB.a/EMS (1), AB.p/P2 (2), and P2/EMS (3) cell boundaries. GPR-1/GPR-2 is enriched two- to threefold at the P2/EMS boundary (E). This enrichment is lost in mes-1(bn74) (G) and src-1(RNAi) (H) embryos but not in mom-5(zu193) (F) embryos. Error bars indicate standard error of the mean. Bar, 10 μm.