Table 3.
Comparison between changes in expression level detected by microarray analysis and real-time RT-PCR
| Transcript
|
Change detected by microarray analysis (fold change)
|
Change detected by real time RT-PCR (fold change)
|
|---|---|---|
| Up-regulated in mammospheres | ||
| Stem cell growth factor | 4.0 | 9.2 |
| HGF antagonist | 149.1 | 132.5 |
| Stem cell growth factor β | 12.9 | 26.5 |
| Apolipoprotein E | 26.9 | 16.9 |
| Up-regulated in differentiated cells | ||
| Parathyroid hormone like hormone | 5.5 | 3.6 |
| TGFα | 6.2 | 2.4 |
| Mammaglobin | 7.4 | 3.2 |
| Neuregulin 1 | Absent in mammospheres, present in differentiated cells |
Absent in mammospheres, present in differentiated cells |
| Similar expression levels (mammospheres vs. differentiated cells) | ||
| LIF receptor | 1.3 | 1.1 |
| LIF | 1.8 | 2.4 |
| Radical fringe | 1.7 | 4.2 |
| Not detected by microarrays (mammospheres vs. differentiated cells) | ||
| Lunatic fringe | Absent in both | 1.15 |
| Manic fringe | Absent in both | 2.08 |
| Notch 4 | Absent in both | 4.1 |
The fold change detected by microarrays represents the average of the two duplicate samples.