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. 1997 Feb 4;94(3):873–878. doi: 10.1073/pnas.94.3.873

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Copyright © 1997, The National Academy of Sciences of the USA

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Immunoblot analysis of Nef-transduced human T cell lines. Cell lysates were prepared from CEM-SS/cat (lane 1), CEM-SS/SIV_mac239 Nef (lane 2), Jurkat (lane 3), Jurkat/SIV_mac239 Nef (lane 4), SupT1 (lane 5), SupT1/SIV_mac239 Nef (lane 6), CEM-SS/HIVfen (lane 7), CEM-SS/HIV-1_NL43 Nef (lane 8), and CEM-SS/HIV-1_cons Nef (lane 9), and resolved on 10% SDS/PAGE (7.5 × 10⁵ cell equivalents per lane). The 32-kDa SIV (lanes 1–6) and 27-kDa HIV (lanes 7–9) Nef isolates were detected by blotting with specific rabbit antisera and visualized with ¹²⁵I-labeled goat anti-rabbit IgG and autoradiography. The 29-kDa molecular weight marker is indicated for each gel.