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Accumulation of internalized CF in cytoplasmic vesicles in control and Nef-expressing CEM-SS cells. HIV-1NL43 Nef-expressing CEM-SS (A) and control CEM-SS/cat (B) cells were incubated with CF for 10 min at 4°C, warmed to 37°C, and incubated overnight. The samples were fixed, sectioned, and examined by EM. G, Golgi complex; M, mitochondria; N, nucleus.
