Table 1.
CD4 surface expression by flow cytometry on control and Nef-expressing human T cell lines
| Cell line | Total mean fluorescence
|
|
|---|---|---|
| P3 | OKT4 | |
| CEM-SS | 4.7 | 188.4 |
| CEM-SS/cat | 5.4 | 105.9 |
| CEM-SS/HIVfen | 10.4 | 178.4 |
| CEM-SS/SIV Nef | 4.7 | 48.5 |
| CEM-SS/HIV-1NL43 Nef | 4.9 | 21.1 |
| CEM-SS/HIV-1cons Nef | 7.0 | 14.0 |
| Jurkat | 5.3 | 40.9 |
| Jurkat/SIV Nef | 3.0 | 16.9 |
| SupT1 | 5.5 | 265.7 |
| SupT1/SIV Nef | 5.0 | 40.9 |
Indirect immunofluorescent staining was performed using the anti-CD4 mAb OKT4 and the mouse IgG1 control mAb P3X63Ag8 (ATCC TIB9; unknown specificity). The results from one representative experiment are shown; staining was repeated with similar results in at least four independent experiments.