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. 2003 Jun 1;17(11):1328–1333. doi: 10.1101/gad.1077103

Figure 1.

Figure 1.

Tankyrase 1-induced telomere elongation is telomerase-dependent. (A–C) Generation of a stable WI38 cell line expressing telomerase. WI38 cells at PD 4 expressing vector control (V) or telomerase (TERT) were analyzed for telomerase expression by immunoblotting whole-cell extracts with anti-TERT 374 antibody (A), or for telomerase activity by TRAP (telomere repeat amplification protocol; B) or for telomere length by Southern blot analysis (C). (D–F) Analysis of stable WI38 cells expressing telomerase (WI38-TERT) and tankyrase 1 alleles. WI38-TERT cells expressing vector control (V), FN-tankyrase1.WT (WT), or FN-tankyrase1.HE/A (HE/A) were analyzed at PD 9 by immunoblotting whole-cell extracts with the antibodies anti-tankyrase 1 376 (TNKS1), anti-poly(ADP-ribose) (PAR), anti-telomerase (TERT), or anti-TRF1 415 (D); or at PD 11 for telomerase activity by TRAP (E); or at the indicated PDs for telomere length by Southern blot analysis (F).