Figure 3.

Defects in pDECAP-Ski transgenic mice is caused by a loss of Ski mRNA. Developmental stage-matched wild-type, pDECAP-β-gal transgenic, pDECAP-Ski transgenic, and Ski-deficient embryos were analyzed. (A) Hematoxylin-eosin staining (H&E) of head sections. (B,C) Ski and Odc mRNA expression in midbrain and mesenchyme as detected by RNA in situ hybridization. For detection of Ski mRNA, the 3′-half of the Ski gene was used as the probe. (D) Apoptotic cells were assessed by the TUNEL assay on the neuroepithelium of midbrain and mesenchyme. (E) H&E staining on eyes. (F) Ski mRNA expression in eyes as detected by in situ hybridization. (G) TUNEL assay on eyes. MB, midbrain; II, second ventricle; III, third ventricle; O, optic cup; L, lens vesicle.