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. 2003 Jun 1;17(11):1352–1365. doi: 10.1101/gad.1089403

Figure 5.

Figure 5.

Bone development in WT, DHet, and DKO embryos. (A) Whole skeletons of E14.5 and E18.5 embryos and newborns (NB) were analyzed for ossification using alcian blue and alizarin red. Ossification is visible in the cranium of E14.5 WT and DHet embryos but not in the cranium of E14.5 DKO embryos. Ossification is nearly complete in WT and DHet newborns, whereas large cartilaginous regions are still present in the cranium and skeleton of DKO newborns. (B) Top view of the cranium (top panel) and side view of the hind limb (bottom panel) of WT and DKO newborns showing cartilaginous regions in the interparietal, exoccipital, and surpraoccipital areas (top panel) and in the femur, tibia, and fibula (bottom panel).