Figure 3.

Confocal images of IAR-2 and C4 cells double labeled for actin filaments (red) and vinculin (green). Areas where actin and vinculin staining overlapped are indicated by the yellow color. (A) Vinculin-positive focal adhesions at the free edge of a wounded IAR-2 cell monolayer did not have any preferential orientation relative to the cell edge. (B) A newly formed contact by four IAR-2 cells. Focal adhesions and straight actin bundles in the contact zone were oriented along the intercellular boundary. Note the concave actin arcs at the edges of the contact. (C) Overlapping C4 cells demonstrated a meshwork of actin filaments and orientation of focal adhesions along the cell axis. (Bar = 10 μm.)