Figure 4.

Confocal images of indirect immunofluorescence staining of E-cadherin (A–C and E) and β-catenin (D and F). Accumulation of E-cadherin in the contact regions at different stages of contact formation and expansion in IAR-2 cells (A–C). Arrow indicates E-cadherin staining in an initial contact (A). Staining of IAR-2 cells for β-catenin showed accumulation of β-catenin in the contact zone (D). C4 cells did not exhibit significant staining for E-cadherin (E). β-Catenin in C4 cells was not enriched in the contact zone (F). (Bar = 10 μm.)