Figure 3.
UV treatment induces the disappearance of Mcl-1 and the translocation of Bcl-xL. (A) HeLa cells left untreated or treated with UV for different amounts of time were either harvested for 1% CHAPS total cell lysate or fractionated into mitochondria (Mito) and S100. The levels of Mcl-1, Bcl-xL, and cytochrome c oxidase were determined in total cell lysate (lanes 1-4), S100 (lanes 5-8), and Mito (lanes 9-12). (B) Human fibroblast cells were treated (as described in Materials and Methods) with UV at different time points before harvest. Mcl-1, Bcl-xL, Bax, and cytochrome c levels were compared in the S100. Mcl-1, Bcl-xL, Bax, and cytochrome c oxidase were compared in the mitochondria.