Figure 2.

Gel electrophoresis and immunoblot analysis of lens proteins. Lens homogenates from αA^+/+, αA^−/−, and αA^+/− mice were separated into soluble and insoluble fractions and subjected to SDS/PAGE. Each lane contains protein from the equivalent of 2.5 μg of lens wet weight. Coomassie staining of the gel (Left) shows the absence of a prominent αA band in the αA^−/− lens (arrowhead). Immunoblot analysis of a duplicate gel with antiserum to recombinant human αA (Right) confirms the absence of αA in the αA^−/− lenses. Bands corresponding to αA and αA^insert are identified. Weak cross-reactivity of this antiserum with a ≈30-kDa lens protein is observed in all three samples.