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Immunoblot analysis of soluble and insoluble lens proteins. Lens homogenates from αA+/+, αA−/−, and αA+/− mice were separated into soluble and insoluble fractions and subjected to immunoblot analysis with a polyclonal antibody to recombinant human αB. Soluble and insoluble proteins representing 2.5 μg of lens wet weight were analyzed.
