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. 2003 Jun 15;17(12):1524–1539. doi: 10.1101/gad.1101503

Figure 3.

Figure 3.

The second peak of Ime2 activity occurs after pachytene. (A,B) Dependence of Ime2 kinase activity and protein on NDT80. Mutant ndt80Δ (KBY430) and wild-type (WT, KBY316) cells were harvested for the Ime2 kinase assay (A) and immunoblotting with 9E10 (B). (C–E) Requirement of Cdc28 for Ime2 activity and for Ime2 and Ndt80 proteins. Mutant cdc28-as1 (KBY442) and wild-type (WT, KBY316) strains were sporulated and treated with 0.5 μM 1-NM-PP1 at 2 h (C,D) or 5 μM 1-NM-PP1 at 0 h (E). Samples were examined by the Ime2 kinase assay (C,E) or immunoblotting to visualize Ime2-myc and Ndt80 (D). (F,G) Modification of untagged Ime2 in wild-type (KBY259), ndt80Δ (KBY427), and cdc28-as1 (KBY439) cells. The cdc28-as1 mutant was treated with 1-NM-PP1 as indicated. Multiple forms of Ime2 are indicated by a column of dots placed between 4and 7 h.