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. 2003 Jun 15;17(12):1524–1539. doi: 10.1101/gad.1101503

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Figure 4. — The ime2-as1 mutation confers sensitivity to 1-NA-PP1. (A) Chemical structures of 1-NA-PP1 and Bn-PP1. (B–D) Wild-type (WT, KBY316) and mutant ime2-as1 (KBY518) cells sporulated in the presence (+) or absence (-) of 20 μM 1-NA-PP1 were harvested for flow cytometry (B), DAPI staining (C), and immunoblotting (D). (C) Meiotic divisions are shown as the percentage of cells with more than one DAPI-staining body. (D) Protein extracts were immunoblotted with antibodies against Ime2-myc (9E10), Sic1, and Ndt80.