Figure 3.

Structure of the mutant tyrosinase gene. (A) Southern blot analysis. Genomic DNA were prepared from C3Hf-c^m1OR/c^m1OR and C3Hf mice, digested with the enzymes indicated, blotted, and hybridized with the tyrosinase cDNA probe, MTY811. (B) Restriction map of pRN209 and pRN210. R, EcoRI; H, HindIII; Hc, HincII. (C) Restriction map of the mutant tyrosinase gene. The solid box on the horizontal line represents the 5.4-kb IAP element, which is inserted head-to-head into the upstream region of the Tyr gene. Boxes below the horizontal line represent the positions of individual probes used in Southern blot and Northern blot analyses. R, EcoRI; H, HindIII; Hc, HincII; B, BamHI; K, KpnI; X, XhoI.