Figure 6.

RT-PCR analysis. (A) Schematic representation of the positions for the primers used in RT-PCR analysis. The +1 represents the tyrosinase major transcription start site. The −56 represents the tyrosinase minor transcription start site (3). The IAP insertion is presented as a solid box. There are two alternatively spliced Tyr transcripts that are different in exon 1 (19). tyr1 contains the full-length of exon 1, tyr2 deletes the shaded box in exon 1. The distance between primers b and c is 878 bp in tyr1 and 647 bp in tyr2. If the tyr transcripts are initiated from IAP promoter in c^m1OR/c^m1OR mice, the expected distance between primers a and c is 1.45 kb in tyr1 and 1.24 kb in tyr2. (B) Total skin RNA, prepared from 4-day-old C3Hf mice and c^m1OR/c^m1OR, respectively, was reverse transcribed in the presence (+) or absence (−) of reverse transcriptase; the resulting cDNA was then amplified by PCR using different primer combinations. The amplified PCR products were electrophoresed, blotted, and hybridized with MTY811.