Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1993 Jan;175(1):7–11. doi: 10.1128/jb.175.1.7-11.1993

Turnover and recycling of the murein sacculus in oligopeptide permease-negative strains of Escherichia coli: indirect evidence for an alternative permease system and for a monolayered sacculus.

J T Park 1
PMCID: PMC196091  PMID: 8416911

Abstract

Turnover of murein in oligopeptide permease-negative Escherichia coli cells appeared to be minimal or nonexistent. In one strain in which it was possible to measure turnover during the first generation of chase, it was found that the rate of turnover was constant throughout a chase of three generations. This result suggests that an "inside-to-outside" mode of growth of the sacculus does not occur in E. coli. Turnover, though minimal, was significantly higher from cells labeled uniformly than from cells labeled only in the lateral wall, suggesting that a significant portion of the observed turnover is related to cell separation. Actually, turnover only appeared to be minimal in opp mutant strains. Tripeptides were being released by turnover at a rate of about 50% per generation and then were efficiently recycled. This suggests that in addition to opp, a low-affinity uptake system for tripeptide derived from the sacculus may exist.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Botta G. A., Park J. T. Evidence for involvement of penicillin-binding protein 3 in murein synthesis during septation but not during cell elongation. J Bacteriol. 1981 Jan;145(1):333–340. doi: 10.1128/jb.145.1.333-340.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Burman L. G., Reichler J., Park J. T. Evidence for multisite growth of Escherichia coli murein involving concomitant endopeptidase and transpeptidase activities. J Bacteriol. 1983 Oct;156(1):386–392. doi: 10.1128/jb.156.1.386-392.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Casadaban M. J., Cohen S. N. Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. J Mol Biol. 1980 Apr;138(2):179–207. doi: 10.1016/0022-2836(80)90283-1. [DOI] [PubMed] [Google Scholar]
  4. Goodell E. W., Higgins C. F. Uptake of cell wall peptides by Salmonella typhimurium and Escherichia coli. J Bacteriol. 1987 Aug;169(8):3861–3865. doi: 10.1128/jb.169.8.3861-3865.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Goodell E. W. Recycling of murein by Escherichia coli. J Bacteriol. 1985 Jul;163(1):305–310. doi: 10.1128/jb.163.1.305-310.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Greenway D. L., Perkins H. R. Turnover of the cell wall peptidoglycan during growth of Neisseria gonorrhoeae and Escherichia coli. Relative stability of newly synthesized material. J Gen Microbiol. 1985 Feb;131(2):253–263. doi: 10.1099/00221287-131-2-253. [DOI] [PubMed] [Google Scholar]
  7. Higgins C. F., Gibson M. M. Peptide transport in bacteria. Methods Enzymol. 1986;125:365–377. doi: 10.1016/s0076-6879(86)25031-4. [DOI] [PubMed] [Google Scholar]
  8. Hobot J. A., Carlemalm E., Villiger W., Kellenberger E. Periplasmic gel: new concept resulting from the reinvestigation of bacterial cell envelope ultrastructure by new methods. J Bacteriol. 1984 Oct;160(1):143–152. doi: 10.1128/jb.160.1.143-152.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Höltje J. V., Glauner B. Structure and metabolism of the murein sacculus. Res Microbiol. 1990 Jan;141(1):75–89. doi: 10.1016/0923-2508(90)90100-5. [DOI] [PubMed] [Google Scholar]
  10. Labischinski H., Goodell E. W., Goodell A., Hochberg M. L. Direct proof of a "more-than-single-layered" peptidoglycan architecture of Escherichia coli W7: a neutron small-angle scattering study. J Bacteriol. 1991 Jan;173(2):751–756. doi: 10.1128/jb.173.2.751-756.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Leduc M., Frehel C., van Heijenoort J. Correlation between degradation and ultrastructure of peptidoglycan during autolysis of Escherichia coli. J Bacteriol. 1985 Feb;161(2):627–635. doi: 10.1128/jb.161.2.627-635.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Leduc M., Fréhel C., Siegel E., Van Heijenoort J. Multilayered distribution of peptidoglycan in the periplasmic space of Escherichia coli. J Gen Microbiol. 1989 May;135(5):1243–1254. doi: 10.1099/00221287-135-5-1243. [DOI] [PubMed] [Google Scholar]
  13. Lutkenhaus J. F., Wolf-Watz H., Donachie W. D. Organization of genes in the ftsA-envA region of the Escherichia coli genetic map and identification of a new fts locus (ftsZ). J Bacteriol. 1980 May;142(2):615–620. doi: 10.1128/jb.142.2.615-620.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mauck J., Chan L., Glaser L. Turnover of the cell wall of Gram-positive bacteria. J Biol Chem. 1971 Mar 25;246(6):1820–1827. [PubMed] [Google Scholar]
  15. Mengin-Lecreulx D., Flouret B., van Heijenoort J. Cytoplasmic steps of peptidoglycan synthesis in Escherichia coli. J Bacteriol. 1982 Sep;151(3):1109–1117. doi: 10.1128/jb.151.3.1109-1117.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Shaw K. J., Berg C. M. Escherichia coli K-12 auxotrophs induced by insertion of the transposable element Tn5. Genetics. 1979 Jul;92(3):741–747. doi: 10.1093/genetics/92.3.741. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wientjes F. B., Pas E., Taschner P. E., Woldringh C. L. Kinetics of uptake and incorporation of meso-diaminopimelic acid in different Escherichia coli strains. J Bacteriol. 1985 Oct;164(1):331–337. doi: 10.1128/jb.164.1.331-337.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wientjes F. B., Woldringh C. L., Nanninga N. Amount of peptidoglycan in cell walls of gram-negative bacteria. J Bacteriol. 1991 Dec;173(23):7684–7691. doi: 10.1128/jb.173.23.7684-7691.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES