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. 1997 Feb 4;94(3):895–900. doi: 10.1073/pnas.94.3.895

Figure 2.

Figure 2

Figure 2

Immunostaining of HoxB9 in combined animal caps. (A and B) FITC fluorescence, staining for HoxB9, and hematoxylin/eosine (HE) staining, as indicated. HoxB9-positive nuclei (arrows) are seen as black holes in the FITC panel because of fluorescence quenching by the immunoprecipitate. (A) An FITC–dextran-injected cap was combined with a cap coinjected with Xlim-1/3m and Xbra mRNAs. (B) A cap coinjected with FITC–dextran and Xlim-1/3m was combined with an Xbra mRNA-injected cap. (C) Frequency of HoxB9 expression in FITC lineage-labeled caps, injected with RNAs as indicated in the figure. n, Number of combined caps examined.