Figure 4.

Evolution of cultured blastocysts and genotyping of cell lines derived from normally and abnormally developing embryos. (A and B) Primary cultures of normally and abnormally developing blastocysts after zona pellucida removal and 5 days of culture, respectively. (C and D) Established cell lines derived from normally and abnormally developing embryos, respectively. (E) Genotyping of the established cell lines by Southern blot analysis following the strategy described in Fig. 1. Lanes 1–4 correspond to cell lines derived from abnormally developing embryos, and lanes 5–8 correspond to lines derived from normal embryos. The 4.2-kb band identifies the mutant allele, and the 3-kb band identifies the wild-type allele.