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. 2003 Jul 1;17(13):1592–1604. doi: 10.1101/gad.1071503

Figure 1.

Figure 1.

(A) Schema for subtractive cDNA cloning of G0 CD34+ CD38– expressed sequences against CD34+ CD38+ cell sequences. (B) cDNA from each round of subtractive hybridization probed with GPR105 (top panel) or GAPDH (bottom panel) demonstrates progressive enrichment of GPR105 and depletion of GAPDH. The lane numbers in the G0 CD34+ CD38– bracket represent the round of subtraction; the lanes in the CD34+ CD38+ bracket represent independent samples. (C) Sequence alignment of human, mouse, and rat GPR105; the amino acid number is indicated. The mouse GPR105 sequence was submitted to the NCBI GenBank (accession no. AF177211).