Figure 4.
Stem cell and radial glia development in embryonic Sox9-deficient spinal cords. (a–j) Immunohistochemistry with antibodies specific for Nkx2.2 and Olig2 (a,f) and in situ hybridizations with antisense probes specific for Olig2, Nkx6.1, and Irx3 (b–d,g–i) were performed on transverse sections from the forelimb region of embryos at 12.5 dpc to determine the integrity of the three ventral stem cell domains of the ventricular zone (p2, pMN, p3) as summarized in e and j. In situ hybridization signals obtained with different probes on immediately adjacent sections were superimposed using Adobe Photoshop with the color of one of the signals being converted to red. (k–p) Immunohistochemistry with antibodies specific for the radial glia marker B-FABP were performed on transverse sections from the forelimb region of embryos. (a–e,k–m) Wild-type (+/+) spinal cords. (f–j,n–p) Sox9Δ/Δ spinal cords. (a–k,n) 12.5 dpc. (l,o) 14.5 dpc. (m,p) 16.5 dpc.