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. 1997 Feb 4;94(3):919–922. doi: 10.1073/pnas.94.3.919

Figure 1.

Figure 1

Northern blot analysis of transgene expression in adipose tissue and muscle of the aP274, aP273, and aP212 lines. Samples from the β-actin promoter–agouti (BAP20) transgenic mice (18) were also included in this analysis. (A) Total RNA (20 μg) from tissues of aP212, aP273, aP274, and BAP20 mice were hybridized with a radiolabeled full-length agouti cDNA probe and, subsequently, with a cDNA probe for glyceraldehyde phosphate dehydrogenase (GAPDH) as a loading control. The same blot was then probed with a cDNA probe for ob (3) to check for adipose tissue contamination.