Figure 2.

(A) Western blots of membrane prepared from GIRK2 +/+, +/−, and −/− mouse brains show that both GIRK2 and GIRK1 protein levels are reduced in the GIRK2 knockout mice. (B) RT-PCR showing equal mRNA expression of GIRK1 in +/+, +/−, and −/− mouse brain. No product is amplified by any of the four pairs of primers in the absence of reverse transcriptase (−/− −RT; lane 4), confirming that all products were amplified from cDNA rather than contaminating genomic DNA. Hypoxanthine phosphoribosyltransferase (HPRT) primers amplify a comparable level of product in all samples, indicating that the same amount of template is present. Amplified products by GIRK1, GIRK4, IRK1, and HPRT primers are of expected sizes.