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. Author manuscript; available in PMC: 2008 Jan 26.
Published in final edited form as: Cell. 2007 Jan 26;128(2):281–294. doi: 10.1016/j.cell.2006.11.049

Figure 2. Phosphorylation by Src reduces p27 inhibitory action on cyclin E-Cdk2.

Figure 2

(A)His-p27WT was phosphorylated in vitro with activated Src. Mock treated (p27) or Src treated pY-p27 were incubated with cyclin E-Cdk2 and H1 kinase activity assayed. Equal input of mock vs. Src treated p27 shown.

(B)His-p27WT incubated without Src (no Src), with inactive Src (dead Src) and active Src are shown. pYp27 was precipitated with αpY-4G10.

Equal amounts of p27 from (B) were incubated with cyclin E-Cdk2. (C) p27, (D) Cdk2 and (E) Cyclin E were precipitated and associated proteins blotted.