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. Author manuscript; available in PMC: 2008 Jan 26.
Published in final edited form as: Cell. 2007 Jan 26;128(2):281–294. doi: 10.1016/j.cell.2006.11.049

Figure 4. cSrc inactivation increases p27 levels and stability and Src induction decreases p27 t1/2.

Figure 4

(A)MCF-7 cells transfected with cSrc siRNA or non-specific controls analyzed for cSrc, 27 and β-actin.

(B)cSrc, p27 and β-actin levels with (+) or without (−) AZD0530 in MDA-MB-361.

(C)Cycloheximide (CHX) chase of p27 in MDA-MB-361 before and 6h after AZD0530.

(D) Src was induced in estrogen starved MCF-pINDSrc2 (E −) by ponasterone A (PA+). 6h after estrogen addition (E+), Src, p27 and β-actin were assayed.

(E) CHX chase of p27 at 6 h after estrogen treatment, with or without prior Src induction.

(F) p27pT187, p27 and β-actin at 13 h after E stimulation, with or without prior Src induction. MG132 added 6 h prior to analysis.