(A)MCF-7 cells transfected with cSrc siRNA or non-specific controls analyzed for cSrc, 27 and β-actin.
(B)cSrc, p27 and β-actin levels with (+) or without (−) AZD0530 in MDA-MB-361.
(C)Cycloheximide (CHX) chase of p27 in MDA-MB-361 before and 6h after AZD0530.
(D) Src was induced in estrogen starved MCF-pINDSrc2 (E −) by ponasterone A (PA+). 6h after estrogen addition (E+), Src, p27 and β-actin were assayed.
(E) CHX chase of p27 at 6 h after estrogen treatment, with or without prior Src induction.
(F) p27pT187, p27 and β-actin at 13 h after E stimulation, with or without prior Src induction. MG132 added 6 h prior to analysis.