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. Author manuscript; available in PMC: 2007 Aug 31.
Published in final edited form as: Methods Mol Biol. 1997;75:153–161. doi: 10.1385/0-89603-441-0:153

Table 1.

Properties of Primary Rabbit Kidney Proximal Tubule Cell Cultures

  1. Morphology (4,10,11)

    Domes

    Form polarized monolayers

    Adjacent cells form tight junctions

    Brush border (although not as elaborated as in vivo)

  2. Transport properties

    Sodium–glucose cotransport system (4,10)

    Sodium–phosphate cotransport system (6)

    p-Aminohippurate transport system (12)

    Amiloride-sensitive sodium transport (13)

  3. Responses to hormones and other effector molecules

    Parathyroid hormone-sensitive cyclic AMP production (4)

    Regulation of phosphate transport by glucocorticoids and estrogens (14,15)

    Regulation of sodium transport by angiotensin II (16)

  4. Enzymes; Metabolic properties

    Leucine aminopeptidase (4)

    Alkaline phosphatase (4)

    Gamma glutamyl transpeptidase (4)

    Glutathione (17,18)

    Glutathione-S-transferase (17,19)

    Angiotensin-converting enzyme (11)

    Phosphoenolpyruvate carboxykinase (20)

    Aerobic metabolism (10)

    Hexose monophosphate shunt (10)

    Gluconeogenesis (21)

  5. Growth properties

    Cell growth in serum-free medium (4)

    Growth in response to insulin, transferrin and hydrocortisone (4,20)

    Growth in response to estrogens (15,22)

    Growth in response to laminin, collagen, and fibronectin

    Growth improved on colloidal silica (9)

    Growth in glucose-free medium (21)

    Can undergo two passages (9)

  6. Responsiveness to toxicants

    Mercury toxicity (17,18)

    Ifosfamide toxicity (23,24)