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(A) Increase in the frequency of KanR mutants in GM31(λKanS)pDCM21 cultures stored at 15°C. (B) Survival of the cultures shown in A. Between days 7 and 53, the average number of KanS per ml decreased from 1.3 to 1 × 109/ml, whereas the average number of KanR per ml increased from 43 to 63.
