Figure 3.

Temporal expression of the chp genes in S. coelicolor grown on solid NMMP medium as determined by Northern analysis. Genes chpE and chpH were expressed after 24 h of growth, when cultures still grew submerged, and during formation of aerial hyphae. In contrast, chpA and chpF were expressed during formation of aerial hyphae only. Expression of chpA was 10- to 25-fold lower compared with that of chpE, chpF, and chpH. Blots were rehybridized with 16S rDNA to confirm that lanes contained equal amounts of RNA.