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Spatial expression of chpC (A) and chpH (B) in S. coelicolor grown on solid medium for 2 d and visualized using eGFP as a reporter. GFP expression was observed in aerial hyphae (AH) of transformants but not in submerged hyphae (SH). Fluorescence due to the chpC promoter was relatively low compared with that of chpH. (C) Wild-type hyphae served as a control. Bar, 10 μm.
