Table 2.

Vectors and constructs used in this study

Plasmid	Description	Reference
pGEM-T	E. coli vector for cloning PCR fragments amplified with TAQ DNA polymerase.	Promega
pGEM-chpA-H	pGEM-T containing a 244-bp internal fragment of chpA, a 377-bp internal fragment of chpB, a 232-bp internal fragment of chpC, the coding sequence of chpD, a 199-bp internal fragment of chpE, a 178-bp internal fragment of chpF, the coding sequence of chpG, and a 119-bp internal fragment of chpH, respectively.	This work
pIJ8630	E. coli-Streptomyces shuttle vector with pUC18 ori and oriT containing an eGFP gene adapted for codon usage in streptomycetes	Sun et al. 1999
pIJ8630-chpA, C, G, H	pIJ8630 containing the 175-bp promoter region of chpA, the 179-bp promoter region of chpC, the 300-bp promoter region of chpG, and the 132-bp promoter region of chpH, respectively, with NdeI sites at the 3′ end allowing translational fusions.	This work