Table 3.
Oligonucleotides used in this study
| Sequence 5′ to 3′ | |
|---|---|
| Gene disruption primers | |
| SC5H1.35c Forward (chpB) | ACTGCCCGCGACCATGTCTGGAAGGCAGGGAGCCTCATGATTCCGGGGATCCGTCGACC |
| SC5H.135c Reverse (chpB) | CCGCCCTGACGGCGCGGGTGCCCCCGGTCGACGCGGTCATGTAGGCTGGAGCTGCTTC |
| SCC46.02c Forward (chpA,D) | CGTCATTCAGGCATGACCGCAGAGAAGGGAAAGTTCATGATTCCGGGGATCCGTCGACC |
| SCC46.01c Reverse (chpA,D) | GGACCGGCCGGGGGCGTCGGGGTGGCGGGGGTGGGGTCATGTAGGCTGGAGCTGCTTC |
| SCI52.16c Reverse (chpC,H) | GGTGCGGAGCCCGCTGCGTGTACCGGTGCGCCCGCCTCATGTAGGCTGGAGCTGCTTC |
| SCI52.17c Forward (chpC,H) | CAATTTTGGGATCGCGTTTGCCAACTAGGAGGAACAATGATTCCGGGGATCCGTCGACC |
| SCI52.17c Reverse (chpH) | CGGCGGAATCAGATCCTCACGGGGCGAGGCACAACGTCATGTAGGCTGGAGCTGCTTC |
| S1 nuclease mapping primers | |
| I52.17c up (chpH) | CGGAGTGGACGAGCGGGTGC |
| I52.17c down IchpH) | GGAAAGGACGCCGGGCGAGTGC |
| SCI5.08c up (chpE) | GCGTGCCCTCGTCCTCTCCG |
| SCI5.08c down (chpE) | CCAGGTTGCCCGAGGCGACG |
| SCC46.03c up (SCO2718) | GTCGTCGCCGATCGCCATCG |
| SCC46.03c down (SCO2718) | CCCGTTGTCGTCCCCGATGG |
| SCD63 up (SCO4772) | GGTGACCGGCCCCTTTCGCG |
| SCD63 down (SCO4772) | GGACGACACCCGCAGTCGGTACG |
| 2SC10A7 up (SCO4002) | GGGAAGAAGGGAAGCAAAGGG |
| 2SC10A7 down (SCO4002) | GCTGCGTGGTGGACGAGTTGC |
| Complementation primers | |
| I52.16c end (chpC) | CGAGTACGGACACTGGGAG |
| I52.17c up (chpH) | see S1 nuclease mapping primers |
| C46 Nhe up | GTCGGCTAGCGCGGCCGGGGCGGGTGTT |
| C46 Nhe down | GTCGGCTAGCGAACCAGCAGTGCGCGGAC |