Gene disruption primers
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SC5H1.35c Forward (chpB)
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ACTGCCCGCGACCATGTCTGGAAGGCAGGGAGCCTCATGATTCCGGGGATCCGTCGACC
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SC5H.135c Reverse (chpB)
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CCGCCCTGACGGCGCGGGTGCCCCCGGTCGACGCGGTCATGTAGGCTGGAGCTGCTTC
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SCC46.02c Forward (chpA,D)
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CGTCATTCAGGCATGACCGCAGAGAAGGGAAAGTTCATGATTCCGGGGATCCGTCGACC
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SCC46.01c Reverse (chpA,D)
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GGACCGGCCGGGGGCGTCGGGGTGGCGGGGGTGGGGTCATGTAGGCTGGAGCTGCTTC
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SCI52.16c Reverse (chpC,H)
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GGTGCGGAGCCCGCTGCGTGTACCGGTGCGCCCGCCTCATGTAGGCTGGAGCTGCTTC
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SCI52.17c Forward (chpC,H)
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CAATTTTGGGATCGCGTTTGCCAACTAGGAGGAACAATGATTCCGGGGATCCGTCGACC
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SCI52.17c Reverse (chpH)
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CGGCGGAATCAGATCCTCACGGGGCGAGGCACAACGTCATGTAGGCTGGAGCTGCTTC
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S1 nuclease mapping primers
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I52.17c up (chpH)
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CGGAGTGGACGAGCGGGTGC
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I52.17c down IchpH)
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GGAAAGGACGCCGGGCGAGTGC
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SCI5.08c up (chpE)
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GCGTGCCCTCGTCCTCTCCG
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SCI5.08c down (chpE)
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CCAGGTTGCCCGAGGCGACG
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SCC46.03c up (SCO2718)
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GTCGTCGCCGATCGCCATCG
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SCC46.03c down (SCO2718)
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CCCGTTGTCGTCCCCGATGG
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SCD63 up (SCO4772)
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GGTGACCGGCCCCTTTCGCG
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SCD63 down (SCO4772)
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GGACGACACCCGCAGTCGGTACG
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2SC10A7 up (SCO4002)
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GGGAAGAAGGGAAGCAAAGGG
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2SC10A7 down (SCO4002)
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GCTGCGTGGTGGACGAGTTGC
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Complementation primers
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I52.16c end (chpC)
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CGAGTACGGACACTGGGAG
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I52.17c up (chpH)
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see S1 nuclease mapping primers
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C46 Nhe up
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GTCGGCTAGCGCGGCCGGGGCGGGTGTT
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C46 Nhe down
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GTCGGCTAGCGAACCAGCAGTGCGCGGAC
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