Figure 5.

Slx1 and Slx4 subunits are required in vitro and in vivo. (A, left) The Y substrate was incubated with 0, 10, 100, or 1000 fmole of Slx1 (Slx1 lanes), Slx4 (Slx4 lanes), Slx1 and Slx4 that had been preincubated on ice for 30 min (Slx1 + Slx4), or the copurified Slx1–4 complex (Complex). The reaction products were then analyzed by native gel electrophoresis. Cleavage products are indicated by a lowercase letter at the right. (Right) Schematic diagram illustrating the sites of cleavage giving rise to the indicated products. (B) Strains of the indicated genotype were spotted in 10-fold serial dilutions on YPD plates or YPD plates containing the indicated concentrations of MMS. The plates were photographed following 3 d of growth at 30°C.