Figure 1.

The pdgf-b^ret allele. (a) Outline of the pdgf-b locus, targeting construct, and Southern identification of the pdgf-b^ret allele. (b) Schematic outline of the pdgf-b^ret allele. Remaining loxP site and inserted TAA-stop denoted by arrowhead and asterisk, respectively. (c) C-terminal sequence of the predicted PDGF-Bwt and PDGF-Bret proteins. (d) Comparison of the retention motifs of mouse PDGF and VEGF members. Basic residues in bold. (e) Northern blot analysis of pdgf-b transcripts in pdgf-b^+/+, pdgf-b^ret/+, and pdgf-b^ret/ret brains. (Left) EtBr-stained gel.